Dr. Osbourne Quaye


Dr. Osbourne Quaye holds PhD in Chemistry (Biochemistry option) from Georgia State University, Atlanta, GA, USA; MSc Chemistry (Biochemistry option) from Georgia State University, Atlanta, GA, USA; M.Phil Biochemistry from University of Ghana, Legon, Accra, Ghana; and B.Sc. Biochemistry and Chemistry from University of Ghana, Legon, Accra, Ghana.

Faculty Profile

Qualifications

  • Ph.D. in Chemistry (Biochemistry Option), Georgia State University, Atlanta, G, USA
  • M.S. in Chemistry (Biochemistry Option), Georgia State University, Atlanta, GA, USA
  • M.Phil. in Biochemistry, University of Ghana, Legon, Ghana
  • B.Sc. in Biochemistry and Chemistry, University of Ghana, Legon, Ghana

Research Interests

  • Zoonotic transmission of gastro-viral agents and the effect of animal gastro-viruses, such as rotavirus, on vaccine development and efficacy
  • Discovery and characterization of medicinal plants as anti-gastro-viral agents
  • Identification and characterization of flavin-dependent enzymes in infectious disease agents as probable/potential drug targets

Professional Experience

2013 – Present | Lecturer, Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon

Teaches Biochemistry, Enzymology, Virology, and Laboratory Techniques; supervises student project work and theses; initiates and supervises research projects.

2009 – 2013 | Guest Research Fellow, Centers for Disease Control and Prevention, Atlanta, GA

National and international rotavirus surveillance using molecular techniques including PCR and nucleic acid sequencing.

2005 – 2009 | Graduate Research and Teaching Assistant, Georgia State University, Atlanta GA

Designed, managed and executed research projects. Analyzedexperimental data and wrote manuscripts for scientific publications.Trained graduate and undergraduatestudents laboratory techniques.Assisted in undergraduate laboratory chemistry courses, and undergraduate and graduate Biochemistry classes.

2002 – 2005 | Research Assistant, Noguchi Memorial Institute for Medical Research (N. M. I. M. R.), University of Ghana, Legon, Accra

Involved in rotavirus studies. Screened samples using ELISA techniques, and identified the type of rotavirus present in the ELISA positive samples using PCR and polyacrylamide gel electrophoresis techniques

2000 – 2001 | Graduate Teaching Assistant, Department of Biochemistry, University of Ghana, Legon

Assisted in undergraduate laboratory courses and proctored undergraduate Biochemistry class tutorial sessions.

Awards

  • Member of the Academic Board of the College of Basic and Applied Sciences
  • Member of the Academic Board of the School of Veterinary Medicine, University of Ghana, Legon
  • Member of the Admissions Board of the School of Biological Sciences, University of Ghana, Legon
  • Fellow of the Cambridge-Africa Partnership for Research Excellence (CAPREx) post-doctoral fellowship
  • Co-Principal investigator of the World Bank funded West Africa Centre for Cell Biology in Infectious Pathogens (WACCBIP) project. Implementation Committee member, and Head of the Monitoring and Evaluation Team
  • Grant awardee of the Cambridge-Africa Alborada Research Fund
  • Seed grant awardee of the Office of Research and Innovation Development (ORID), University of Ghana, Legon
  • External Examiner of the Diarrhoeal Pathogens Research Unit, University of Limpopo, Medunsa, South Africa
  • Internal Examiner of the School Graduate Studies, University of Ghana, Legon
  • Reviewer of the Pediatric Infectious Disease Journal
  • Course Advisor (Level 300) at the Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon
  • Figure from publication was made the cover-page of the April-June 2008 issues of Biochemistry, a journal of the American Chemical Society
  • Chair’s Award for Outstanding Research at the Graduate Doctoral Level in 2008, Department of Chemistry, Georgia State University

Publishing Portfolio

Mijatovic-Rustempasic, S., Tam, K. I., Kerin, T. K., Lewis, J. M., Gautam, R., Quaye, O., Gentsch, J. R. and Bowen, M. D. (2013); Sensitive and Specific Quantitative Detection of Rotavirus A by One-Step Real-Time Reverse Transcription PCR Assay without Antecedent dsRNA Denaturation; Journal of Clinical Microbiology, 51(9): 3047-3054.

Quaye, O., McDonald, S., Esona, M., Lyde, F., Gentsch, J. and Bowen, M. (2013); Detection of rotavirus G9P[4] strains in three Latin America Countries; Surveillance during the 2009-2010 rotavirus season; EmergingInfectious Diseases, 19(8): 1332-1333.

Gautam, R., Lyde, F., Esona, M. D., Quaye O., and Bowen, M. D.(2013); Comparison of PremierTMRotaclone®, ProSpecT™, and RIDASCREEN® Rotavirus Enzyme Immunoassay Kits for Detection of Rotavirus Antigen in Stool Specimens; Journal of Clinical Virology, 58(1): 292-294.

Patel, M.M., Patzi, M., Pastor, D., Nina, A., Roca, Y., Alvarez, L., Iniguez, V., Rivera, R., Tam, K.I., Quaye, O., Bowen, M., Parashar, U. and De Oliveira, L.H. (2013); Effectiveness of monovalent rotavirus vaccine in Bolivia: case-control study; British Medical Journal (Web Published).

Cortese, M.M., Immergluck, L.C., Held, M., Jain, S., Chan, T., Grizas, A.P., Khizer, S., Barrett, C., Quaye, O., Mijatovic-Rustemapasic, S., Gautam, R., Bowen, M.D., Moore, J., Tate, J.E., Parashar, U.D. and Vázquez, M.(2013) Effectiveness of monovalent and pentavalent rotavirus vaccines; Pediatrics, 132(1): 25-33.

Esona, M.D., Mijatovic-Rustempasic, S., Foytich, K., Roy, S., Banyai, K., Armah, G., Steele, A., Volotão, E., Gomez, M., Silva, M., Gautam, R., Quaye, O., Tam K, Forbi J, Seheri M, Page N, Nyangao J, Ndze V, Aminu M, Bowen M and Gentsch J. (2013); Human G9P[8] rotavirus strains circulating in Cameroon, 1999-2000: genetic relationships with other G9 strains and detection of a new G9 subtype; Infection, Genetics and Evolution,18: 315-324.

Cardemil, C. V., Cortese, M. M., Medina-Marino, A., Jasuja, S., Desai, R. Leung, J., Rodriguez-Hart, C., Villaruel, G., Howland, J., Quaye, O., Tam, K. I., Bowen, M. D., Parashar, U., Gerber, S. and the Rotavirus Investigation team (2012); Two rotavirus outbreaks caused by genotype G2P[4] at large retirement communities; Annals of Internal Medicine,157(9): 621-631.

Boom, J. A., Tate, J. E., Sahni, L. C., Rench, M. A., Quaye, O., Mijatovic-Rustempasic, S., Patel M. M., Baker, C. J.and Parashar, U. D. (2010); Sustained protection from pentavalent rotavirus vaccination during the second year of life at a large, urban US pediatric hospital; Pediatric Infectious Disease Journal, 29(12) : 1133-1135.

Quaye, O., Nguyen, T. Gannavaram, S., Pennati, A. and Gadda, G. (2010); Rescuing of the hydride transfer reaction in the Glu312Asp variant of choline oxidase by a substrate analogue;Archives of Biochemistry and Biophysics, 499(1-2): 1-5.

Quaye, O. and Gadda, G. (2009); Effect of a conservative mutation of an active site residue involved in substrate binding on the hydride tunneling reaction catalyzed by choline oxidase; Archives of Biochemistry and Biophysics, 489(1-2): 10-14.

Quaye, O., Cowins, S. and Gadda, G. (2009); Contribution of flavin covalent linkage with His99 to the reaction catalyzed by choline oxidase; Journal of Biological Chemistry, 284(25): 16990-16997.

Gadda, G., Pennati, A., Francis, K., Quaye, O., Yuan, H., Rungsrisuriyachai, K., Finnegan, S., Mijatovic, S., Nguyen, T. (2008); Hydride transfer made easy in the oxidation of alcohols catalyzed by choline oxidase; 16th International Symposium on Flavins and Flavoproteins, Jaca, Spain.

Quaye, O., Lountos, G. T., Fan, F., Orville, A. M. and Gadda, G. (2008); Role of Glu312 in binding and positioning of the substrate for the hydride transfer reaction in choline oxidase; Biochemistry, 47(1): 243-256.

Manuscripts in Preparation

Quaye, O.; Xu, Z.; Tam, K.I.; Esona, M.D.; Roy, S.; Gentsch, J.R. and Bowen, M.D. (2014); Characterization of a G10P[14] Human rotavirus strain detected in a sample from Honduras collected during the 2010-2011 rotavirus season; Journal of Infectious Diseases (proposed).

Quaye, O., Hay, S., Pudney, C., Scrutton, N. and Gadda, G. (2014); Probing the mechanism of hydride ion transfer in choline oxidase variant enzymes; Biochemistry (proposed).

Research Reports

Quaye, O. (2009); On the role of pre-organization of the active site of choline oxidase for hydride transfer and tunneling mechanism; Dissertation submitted to the Department of Chemistry, Georgia State University, Atlanta, GA, in partial fulfillment of the requirement for a Doctor of Philosophy Degree in Chemistry (Biochemistry option).

Quaye, O. (2007); Role of Glu312 in binding and positioning of the substrate for hydride Transfer Reaction in Choline Oxidase; Thesis submitted to the Department of Chemistry, Georgia State University, Atlanta, GA, in partial fulfillment of the requirements for a Master of Science Degree in Chemistry (Biochemistry option).

Quaye, O. (2001); Biochemical toxicology of Desmodium adscendens; thesis submitted to the Department of Biochemistry, University of Ghana, Legon, in partial fulfillment of the requirements for a Master of Philosophy Degree in Biochemistry.

Quaye, O. (1998); Fractionation of a crude extract from Bridelia ferruginea, an anti-diabetic plant, using chromatographic techniques; Dissertation submitted to the Department of Biochemistry, University of Ghana, Legon, in partial fulfillment of the requirements for the award of a Bachelor of Science Degree in Biochemistry.

Presentations

Oral Presentations

Quaye, O. (2013); On the story of active site pre-organization in choline oxidase: rescue of Glu312Asp variant with substrate analogue; Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon; 11/03/2013.

Quaye, O. (2012); Rotavirus surveillance in the post-vaccine introduction era: results from the Americas (2009-2010); Department of Biochemistry, Cell and Molecular Biology, University of Ghana, Legon; 10/09/2012.

Quaye, O. (2010); Introduction to rotaviruses and rotavirus sample preparation; “Testing methods for vaccine surveillance and control of rotavirus gastroenteritis”; Centers for Disease Control and Prevention (CDC), Atlanta, GA; 09/28/2010 to 09/30/2010.

Gadda, G. and Quaye, O. (2008); Does flavin covalent linkage to the protein affect preorganization of the enzyme-substrate complex in choline oxidase?; Seminar Series of the Department of Chemistry, University of South Florida, Tampa, FL; 09/11/2008.

Gadda, G. and Quaye, O. (2008); On the importance of enzyme-substrate preorganization in the hydride ion tunneling reaction catalyzed by choline oxidase; 84th Annual American Chemical Society Florida Section Meeting (FAME), Orlando, Florida; 05/08/2008 to 05/10/2008.

Quaye, O. (2007); Role of Glu312 in binding and positioning of the substrate in the reaction catalyzed by choline oxidase; Seminar Series of the Department of Biochemistry, Cell & Molecular Biology, University of Ghana, Legon; 10/05/2007.

Quaye, O. (2007); On the role of Glu312 in the binding and correct positioning of substrate for efficient catalysis in choline oxidase; Atlanta Flavin Meeting Presentation Series, Atlanta, GA; 02/01/2007.

Quaye, O. (2004); Acute and sub-chronic toxicity studies of an anti-asthmatic and anti-inflammatory plant medicine AN2000; 4th Annual Research Meeting, “Bridging the Research-Policy Divide”, Noguchi Memorial Institute for Medical Research, University of Ghana, Legon; 11/22/2004 to 11/24/2004.

Numerous presentations as a graduate student at Georgia State University and Guest Research Fellow with the Rotavirus Surveillance Team at the Centers for Disease Control and Prevention (CDC).

Poster Presentations

Quaye, O.; Hay, S., Pudney, C., Scrutton, N. and Gadda, G. (2013); Probing the mechanism of hydride ion transfer in choline oxidase variant enzymes; 4th Southeastern Enzyme Conference, Atlanta, GA, USA; 04/19/2013.

Quaye, O.; Xu, Z.; Tam, K.I.; Esona, M.D.; Roy, S.; Gentsch, J.R. and Bowen, M.D. (2012); Characterization of a G10P[14] Human rotavirus strain detected in a sample from Honduras collected during the 2010-2011 rotavirus season; 10th International Rotavirus Symposium, Bangkok, Thailand; 09/19/2012 to 09/21/2012.

Quaye, O.; McDonald, S.; Lyde, F.; Esona, M.D.; Gentsch, J.R. and Bowen, M.D. (2011); Detection of G9P[4] strains in three Latin America countries during the 2009-2010 rotavirus season; 30thAmerican Society of Virology, Minneapolis, MN; 07/16/2011 to 07/20/2011.

Quaye, O.; Cowins, S. and Gadda, G. (2008); Contribution of FAD C8M covalent linkage with His99 to the reaction catalyzed by choline oxidase; 2nd International Interdisciplinary Conference on Vitamins, Coenzymes and Biofactors, Athens, GA; 10/26/2008 to 10/31/2008.

Gadda, G.; Finnegan, S.; Nguyen, T.; Quaye, O.; Rungsrisuriyachai, K.; Yuan, H. and Orville, A. M. (2007); Preorganization of the enzyme-substrate complex in the reaction of alcohol oxidation catalyzed by choline oxidase; Gordon Research Conference, “Enzymes, Coenzymes and Metabolic Pathways”; University of New England, Biddeford, ME; 07/08/2007 to 07/13/2007.

Gadda, G.; Pennati, A.; Francis, K.; Quaye, O.; Yuan, H.; Rungsrisuriyachai, K.; Finnegan, S.; Mijatovic, S.; Nguyen, T. and Orville A.M. (2008); Hydride transfer made easy in the oxidation of alcohols catalyzed by choline oxidase; Proceedings of the 16th International Symposium, “Flavins and Flavoproteins 2008”, Jaca, Spain;06/08/2008 to 06/13/2008.

Quaye, O. and Gadda, G. (2007); On the role of Glu312 in the binding and correct positioning of substrate for efficient catalysis in choline oxidase; 15th Annual Bud Suddath Bioscience Symposium; “Directed Molecular Evolution Mechanisms”; Georgia Institute of Technology, Atlanta, GA; 03/30/2007 to 03/31/2007.

Quaye, O. and Gadda, G. (2007); On the role of Glu312 in the binding and correct positioning of substrate for efficient catalysis in choline oxidase; 20th Enzyme Mechanisms Conference; St. Petersburg Beach, FL; 01/03/2007 to 01/06/2007.

Quaye, O. and Gadda, G. (2006); On the mechanistic importance of Glu312 residue in choline oxidase; 2nd Annual Prostate Cancer Symposium, Clark Atlanta University, Atlanta GA; 03/30/2006 to 03/31/2006.

Quaye, O. and Gadda, G. (2006); On the mechanistic importance of active site residue Glu312 in choline oxidase; 3rd Annual SER-CAT Symposium; “Interesting Structures and Advances in SER-CAT Facilities”, Georgia State University, Atlanta, GA; 03/10/2006.

Teaching & Research

Centre for Biotechnology and Genomic Research

The centre engages in contemporary research at the interface of discipline constituting biotechnology in its broadest definition. A broad range of research areas includes:

Biomedical Sciences | Molecular and Cellular Biology | Biophysics | Bioengineering and Devices | Biochemistry | Biotechnology | Regulatory Affairs | Climate Sciences, Agriculture and Environment

Microbiology Consulting Group

The group provides expertise microbiology consultancy, pharmacopoeia compliance, contamination control, and training solutions for biotechnology, pharmaceutical, and health related industries within Ghana.